Abstract
To determine the radioactivity in the carboxyl group of amino acids, the automatic amino acid analyzer was used in conjunction with a scintillation counter. The radioactivity in each amino acid was determined using a flow cell in the scintillation counter before the ninhydrin reaction and discrete samples were collected in a fraction collector after the ninhydrin reaction. The loss in radioactivity from before to after the ninhydrin reaction was shown to have been due to decarboxylation. The information was used to study amino acid biosynthesis in the spirochete Leptospira.
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