Abstract

A GC-MS-Selected Ion Monitoring (SIM) detection method was developed for simultaneous determination of four monoterpenes: (−)-menthone, (+)-pulegone, (−)-limonene and (+)-menthofuran as the main bio-active and toxic constituents, and four other main compounds in the volatile oils of Schizonepeta tenuifolia (ST) leaves and spikes at different harvesting times. The results showed that the method was simple, sensitive and reproducible, and that harvesting time was a possible key factor in influencing the quality of ST leaves, but not its spikes. The research might be helpful for determining the harvesting time of ST samples and establishing a validated method for the quality control of ST volatile oil and other relative products.

Highlights

  • The dried aerial part of Schizonepeta tenuifolia (Benth.) Briq. (ST, called Jingjie in China) has long been popularly employed in mainland China, Hong Kong, Taiwan and Japan as a traditional medicinal herb for the treatment of colds, headaches, fevers, skin rashes andInt

  • The aerial parts of Schizonepeta tenuifolia were obtained at seven different harvesting times from a Good Agriculture Practice (GAP)-based herbal garden which is located in Anguo, Hebei province

  • All the main components were separated completely in 35 min, and the eight compounds for quantification were identified on the basis of comparison of their mass spectra with NIST05 database through MSD

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Summary

Introduction

The dried aerial part of Schizonepeta tenuifolia (Benth.) Briq. (ST, called Jingjie in China) has long been popularly employed in mainland China, Hong Kong, Taiwan and Japan as a traditional medicinal herb for the treatment of colds, headaches, fevers, skin rashes (pruritus and rubella) andInt. The dried aerial part of Schizonepeta tenuifolia (Benth.) Briq. (ST, called Jingjie in China) has long been popularly employed in mainland China, Hong Kong, Taiwan and Japan as a traditional medicinal herb for the treatment of colds, headaches, fevers, skin rashes (pruritus and rubella) and. Much research regarding pharmacological actions in vivo and in vitro has shown its extracts (aqueous or methanolic) to have various biological and pharmaceutical properties, including antipruritic [2], hemostatic activity [3,4], and effects on the immune system [5,6]. The volatile components were recognized as the major constituents responsible for its biological effects. The volatile oil distilled from its aerial part and spike showed potent anti-inflammatory [7] and fumigant activity [8,9,10]

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