Abstract
The characterisation and quantitative analysis of lipids in foods relies on the combination of gas-liquid chromatography (GLC) and adsorption chromatography. Despite the exceptional degree to which GLC has been developed, quantitative problems still exist, particularly in the analysis of triglycerides and diglycerides. Quantitative analysis of lipids on thin-layer chromatograms has been tried by densitometric scanning, with mixed success. Flame ionisation scanning of “Chromarods” using the Iatroscan THIO yields better results, but considerable errors still exist. It is preferable to substitute high-performance liquid chromatography for thin-layer chromatography where accuracy is required. However, the lack of a suitable, commercially available detector system is restrictive. Nevertheless, excellent results are obtained using the Pye Unicam LCM2 transport detector.
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