Abstract

Polarimetry is sensitive to the microstructures and has its advantages in characterizing the tissue microstructures. Our previous work on dynamic polarization changes with tissue clearing has investigated the tissue variations by using glycerol,saturated sucrose and formamide.In this study, we use a fast Mueller matrix microscope to quantitatively compare the different mechanisms of two clearing agents: glycerol and formamide. We obtain the temporal Mueller matrix images with an interval of 15 seconds and extract the temporal features of polarization parameters. Except the depolarization and linear retardance, we also analyze Mueller matrix transformation (MMT) parameters and rotation invariants derived from Mueller matrices. The former is used to characterize the microscale anisotropy of scattering media, and the latter is suitable for assessing the breaking of symmetries of scattering matrix. These Mueller matrix parameters convey the micro characteristic of biological tissues, and then help to explore and explain the microstructural changes of tissue properties by optical clearing.

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