Quantitative characterisation of Plasmodium vivax in infected erythrocytes: a textural approach

Abstract

This paper aims at introducing a textural pattern analysis approach to Plasmodium vivax P. vivax detection from Leishman stained thin blood film. This scheme follows retrospective study design protocol where patients were selected at random in the clinic. The scheme consists of four stages - artefacts reduction, fuzzy divergence-based segmentation of P. vivax infected regions and normal erythrocytes, textural feature extraction using grey level co-occurrence matrix and fractal dimension, finally classification. Here, we have extracted seven features, out of which five are statistically significant in discriminating textures between malaria and normal classes based on light microscopic blood images at 100× resolutions. Finally, Bayesian and support vector machine-based classifiers are trained and validated with 100 cases and 100 control subjects. In effect, it is hereby observed that the significant textural features lead to discriminate P. vivax with 95% and 98% accuracies for SVM and Bayesian classifiers respectively. Results are studied and compared.