Abstract
The surface microlayer of two small ponds in Wisconsin were studied from March 1979 through November 1979 using glass plate and screen microlayer sampling devices. The numbers of fungal colony-forming units (CFU) in the surface microlayer were determined and compared with numbers in subsurface waters; diel fluctuations were correlated with nutrients; and experiments were conducted to estimate the contribution of spores to surface microlayer populations. The data obtained indicates that the highest number of fungal CFU were located in the surface microlayers of the ponds studied. The numbers present, as well as their enrichment in the surface microlayer, underwent both seasonal and diel fluctuations. Most of the fungal CFU in the surface microlayer appeared to be spores arriving from both allochthonous and autochthonous sources. Qualitative investigations would be necessary to determine the relative importance of either source to the total numbers of fungi observed.
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