Abstract
A HPLC method for separation and quantitative determination of secoiridoid glucosides in fruit extracts from LIGUSTRUM VULGARE L. is described. A system using a reversed phase column and an ethanol-water gradient elution, was suitable for separation of Ligstroside, Oleuropein, Nüzhenide and LF (3) 8. The secoiridoid glucosides were detected at 235 nm. The detection limits were within 2 and 10 microg/ml. Ripe fruits yield about 8.85% of secoiridoid glucosides (dried weight).
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