Quantitative autoradiographic analysis of the distribution of [ 3H]muscimol binding to GABA receptors in chick brain

Abstract

Quantitative receptor autoradiography was used to investigate the distribution of high-affinity GABA receptors (GABA A) in left and right hemispheres of the brains of 3-week-old chicks. The receptors were labelled with the potent GABA agonist [ 3H]muscimol. High levels of [ 3H]muscimol labelling were found throughout the fore-, mid-, and hindbrain, though considerable variation was found in different regions. In the telencephalon the highest concentration of specific binding was found in the hyperstriatum ventrale followed by the neostriatum, and then the locus parolfactorius of the paleostriatal complex, whilst in the diencephalon highest levels of labelling were present in the infundibulum. In the midbrain distinct lamination was observed in the high levels of [ 3H]muscimol binding in the optic tectum and in the hind brain the highest density of labelling occurred in the granular layers of the cerebellum. Levels of labelling were generally low in the brainstem regions. The distribution of [ 3H]muscimol binding sites in good agreement with our previous work on the distribution of GABA-immunoreactivity in the chick brain.