Quantitative assessment of the infection rate of the entomophthoraceous fungus,Zoophthora anhuiensis against the green peach aphid Myzus persicae

Abstract

A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus,Zoophthora anhuiensis (Li) Humber, on the green peach aphid,Myzus persicae (Sulzer). Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4–10.4 conidia/mm2) ofZ. anhuiensis F97028, was used to yield an estimate of expected mortality probability at a given dosage. Secondly, biossay 2 was conducted by simultaneously exposing six ≤4-day-old nymphal colonies to a shower ofZ. anhueinsis conidia at each of four dosages (resulting from exposures of 0.3–8.0 min). Subsequently, the colonies were separately immersed in a 0.1% chlorothalonil solution for 0.5 min to disinfect all surviving conidia on the host integument from 1–12 h after exposure under temperature treatments of 15 and 20°C, respectively. The infection rate during a specific period from the end of the exposure to the immersion was then estimated as the ratio of the observed mortality over the expected mortality probability at a particular dosage. The results showed that the infection ofM. persicae fromZ. anhuiensis was highly rapid with little difference between aphid colonies maintained at 15 and 20°C before being immersed in the fungicidal solution after exposure. The first 6-hour period after exposure was most crucial to successful infection of the fungus with the infection rate greatly depending on conidial dosages. It took ≤1 h to infect >50% of the aphids at a dosage of >1.5 conida/mm2 and >90% at >50 conidia/mm2.