Abstract
A portable, quantitative, and selective DNA detection biosensor, based on a loop-based DNA competitive hybridization assay and a personal glucose meter (PGM), is an advanced strategy for one-step target DNA recognition and signal reporter generation. In the presence of target DNA, the invertase-DNA conjugates are released due to the competitive binding of target DNA and collected with the help of a magnet subsequently. The released invertase-DNA could catalyze the hydrolysis of sucrose into glucose with millions of turnovers which is target concentration dependent. In addition, the sensor exhibits excellent anti-interference ability, having almost no effect on the detection performance in serum. The biosensor shown here is easier to operate owning its great potential in point of care testing in environments with limited resources and skilled personnel for rapid and sensitive detection of specific DNA sequence in real biological samples.
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