Abstract
A simple and reliable method of high-performance liquid chromatography with photodiode array detection (HPLC-DAD) was developed to evaluate the quality of Receptaculum Nelumbinis (dried receptacle of Nelumbo nucifera) through establishing chromatographic fingerprint and simultaneous determination of five flavonol glycosides, including hyperoside, isoquercitrin, quercetin-3-O-β-d-glucuronide, isorhamnetin-3-O-β-d-galactoside and syringetin-3-O-β-d-glucoside. In quantitative analysis, the five components showed good regression (R > 0.9998) within linear ranges, and their recoveries were in the range of 98.31%–100.32%. In the chromatographic fingerprint, twelve peaks were selected as the characteristic peaks to assess the similarities of different samples collected from different origins in China according to the State Food and Drug Administration (SFDA) requirements. Furthermore, hierarchical cluster analysis (HCA) was also applied to evaluate the variation of chemical components among different sources of Receptaculum Nelumbinis in China. This study indicated that the combination of quantitative and chromatographic fingerprint analysis can be readily utilized as a quality control method for Receptaculum Nelumbinis and its related traditional Chinese medicinal preparations.
Highlights
Lotus (Nelumbo nucifera Gaertn.), belonging to the family Nymphaeaceae, is a kind of perennial aquatic herbage plant, which is one of the most important aquatic vegetables widely grown in China, due to its pleasant flavor and high nutritional value, especially its seeds, rhizomes and leaves
A HPLC fingerprint and quantitative analysis method was developed to evaluate the quality of Receptaculum Nelumbinis from different sources
The method was well validated by systematically comparing chromatograms of all samples from different sources and certified helpfully to improve the quality control
Summary
Lotus (Nelumbo nucifera Gaertn.), belonging to the family Nymphaeaceae, is a kind of perennial aquatic herbage plant, which is one of the most important aquatic vegetables widely grown in China, due to its pleasant flavor and high nutritional value, especially its seeds, rhizomes and leaves. Up to the year 2002, a total of 572 lotus accessions (including landraces, cultivars and breeding lines) with different germplasm resources were conserved in the National Garden of Aquatic Vegetable in Wuhan, Hubei province, China, including those collections from 153 counties in 18 provinces and lines bred by breeders. Rhizome lotus is mainly cultivated in Hubei, Jiangsu, Anhui and Zhejiang provinces, seed lotus in Jiangxi, Fujian and Hunan and flower lotus in Wuhan city, Hubei province and Beijing [2].
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