Abstract
In order to improve the evaluation and to reduce the measuring error of stained or coloured thin-layer chromatoplates, a two-dimensional image-processing procedure has been used. The complete area of the stained spots and their total absorption were measured automatically. The procedure is especially useful for the analysis of asymmetric or distorted spots and provides complete information. For data acquisition, a computer-controlled high-speed scanning photometer was used in a vertical remission mode. The computer software for the digital evaluation of variously stained chromatoplates is described, and the flexibility of the method demonstrated with some typical examples. The detection limit for stained spots is in the nanogram range and the mean error is below 2%.
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