Abstract
BackgroundPueraria lobata flower (Gehua) is a medicinal herb to treat intoxication, hepatic and gastrointestinal tract lesion induced by alcohol. This study aims to develop a new HPLC method for the determination of two major isoflavones in P. lobata flowers, namely tectoridin and 6"-O-xylosyl-tectoridin.MethodsA high performance liquid chromatography (HPLC) method with a C18 column (250 mm × 4.6 mm, 5 μm) was developed for the quantitative analysis of tectoridin and 6"-O-xylosyl-tectoridin, the main isoflavone components in P. lobata flower. A simple gradient of acetonitrile/water (0 min 15:85; 35 min 50:50; 36 min 15:85; 40 min 15:85; v/v) was used, and 265 nm was selected as detection wavelength. Tectoridin and 6"-O-xylosyl-tectoridin were used as the external standards in quality control of P. lobata flower for the first time. The method was applied to practical use in quality assessment of eleven batches of P. lobata flower samples in Chinese herbal medicine market.ResultsThe peak area response was linear for tectoridin in the 11.8-236.4 μg/mL range with a correlation coefficient of 0.9996 (P < 0.001), and for 6"-O-xylosyl-tectoridin in the 10.33-185.99 μg/mL range with a correlation coefficient of 0.9984 (P < 0.001) respectively. The average recoveries were 102.7-103.7% for tectoridin and 95.7-103.2% for 6"-O-xylosyl-tectoridin (RSDs < 3%), and the intra-day and inter-day RSDs of the two components were less than 2%. This HPLC method was applied to assess the quality of P. lobata flower from eleven provinces in China. P. lobata flowers from northern China contained 26.46-43.28 mg/g of tectoridin and 30.90-48.23 mg/g of 6"-O-xylosyl-tectoridin comparing to 10.00-19.81 mg/g of tectoridin and 11.08-37.03 mg/g of 6"-O-xylosyl-tectoridin in those from southern China.ConclusionThe results showed that P. lobata flowers from northern China contained more tectoridin and 6"-O-xylosyl-tectoridin than those from southern China.
Highlights
Pueraria lobata flower (Gehua) is a medicinal herb to treat intoxication, hepatic and gastrointestinal tract lesion induced by alcohol
Most of pharmacological effects of P. lobata flower have been attributed to its isoflavone components [2,3,5,6,8,9,10], e.g. kakkalide, kakkalidone, puerarin, irisolidone, 6"-O-xylosyl-glycitin, tectoridin and 6"-Oxylosyl-tectoridin (Figure 1); determination of isoflavone is crucial for the quality control of P. lobata flower [11]
We found that tectoridin was the major component of ethanol (70%) extract of P. lobata flower
Summary
Pueraria lobata flower (Gehua) is a medicinal herb to treat intoxication, hepatic and gastrointestinal tract lesion induced by alcohol. This study aims to develop a new HPLC method for the determination of two major isoflavones in P. lobata flowers, namely tectoridin and 6"-O-xylosyl-tectoridin. While the P. lobata root (Gegen) is beneficial for cardiovascular diseases, the P. lobata flower (Gehua) is used to treat intoxication, hepatic and gastrointestinal (GI) tract lesion induced by alcohol [1]. P. lobata flower reduces ethanol absorption by the GI tract [2,3] and modulates the immune and endocrine systems to alleviate the damage caused by alcohol to the hepatic and GI functions. We found that tectoridin was the major component of ethanol (70%) extract of P. lobata flower. Tectoridin is used as BioMed Central tribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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