Abstract
A method is described for the quantitative separation of the neutral lipid fraction of carrageenin granulomas into the major neutral lipid classes, i.e., monoglycerides, diglycerides, triglycerides, free fatty acids, sterols, sterol esters, and hydrocarbons, using the technique of thin-layer chromatography. The radioactivity and lipid content found in the total neutral lipid fraction were quantitatively recovered in the separated neutral lipid classes. Although the method described was applied to the separation of the neutral lipid from carrageenin granulomas for studies of lipid changes during connective tissue growth, the results demonstrate that thin-layer chromatography can be applied successfully in the quantitative separation and recovery of the major neutral lipid classes of tissue particularly when a complete survey of the lipid content and incorporation of radioactive tracers into the neutral lipid classes of tissue is desired.
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