Quantitative Analysis of the Interfacial Barrier to Membrane Transport of Cholesterol Solubilized in a Charged Micellar System

Abstract

The transport of cholesterol solubilized in polyoxyethylene(10)-nonylphenol ether with benzyldimethyltetradecylammonium chloride additive (4:1, w/w) was studied in a diffusion cell separated by a silicone rubber membrane. Overall kinetics revealed the presence of an interfacial barrier. Additions of NaCl or Na2SO4 abolished the barrier to the extent that total cholesterol flux was essentially limited by membrane diffusion considerations. The results are consistent with the concept of micelles diffusing in an electrical field followed by a collision-complex transfer of cholesterol in the aqueous–membrane interfacial region. The electrostatic force of repulsion arises from the overlap of diffuse electrical double layers emanating from the charged mixed micelle and the cationic surfactant adsorbed on the membrane. The influence of surfactant concentration on cholesterol transport kinetics was consistent with electrostatic phenomena. The derived physical model focused on interfacial electrical properties in the donor chamber by maintaining a high concentration (≥0.1 M) of strong electrolyte in the receiver. A linear regression of the logarithm of theoretical transport resistance, total resistance less membrane and receiver boundary layer resistances, versus (ionic strength)1/2 in the donor resulted in a reasonable estimate of the total surface potential of the micelle and membrane surfaces as well as the net dispersion attraction constant.