Quantitative analysis of the combined effects of temperature, evaporative demand and light on leaf elongation rate in well-watered field and laboratory-grown maize plants

Abstract

The respective effects of meristem temperature, vapour pressure deficit (VPD) and photosynthetic photon flux density [PPFD) on leaf elongation rate (LER) of maize, in the absence of water deficit in the soil have been quantified. This analysis was carried out in a series of field experiments in northern and southern France over several seasons and years, and in growth chamber experiments. LER was measured with 10 min steps, together with meristem temperature, VPD and PPFD at leaf level in three types of experiments: in growth chamber experiments with steps in PPFD or VPD at constant meristem temperature, in growth chamber experiments with several combinations of constant, but contrasting, PPFDs, VPDs and meristem temperatures, and in the field with fluctuating conditions, (i) When evaporative demand was low (night or day with low air VPD), LER was only linked to meristem temperature, regardless of other climatic conditions, (ii) Light had no effect per se on LER in the range from 0 to 1500 ftmo m~2 s~1 for time-scales longer than 2 h, provided that its indirect effects on meristem temperature and on evaporative demand were corrected (in the growth chamber) or taken into account (in the field), and provided that cumulated PPFD over a weekly time-scale was compatible with field conditions, (iii) Evaporative demand sensed by growing leaves, as estimated by meristem-to-a ir vapour pressure difference, markedly affected LER in the range from 1-4 kPa, at all time-scales under study, with a unique relationship in the growth chamber (constant conditions) and in the field (fluctuating conditions). This effect was only observed when PPFD was high enough for stomata to open. The negative effect of evaporative demand on LER was probably not due to long distance root-to-shoot signalling, since soil was wet, calculated root water potential remained close to 0 MPa and concentration of ABA in the xylem sap was very low. Therefore, it is proposed to model maize LER with a two-step process, involving the calculation of the maximum LER at a given meristem temperature and then the calculation of the reduction in LER due to evaporative demand. Joint analysis of the whole set of data by using the two equations yielded a r2 of 0.75. This twostep process would be more accurate than the provision of LER from temperature only in cases where air VPD frequently exceeds 2 kPa.