Quantitative analysis of synthetic mixtures of triacylglycerols with fatty acids from caprylic to stearic

Abstract

Separation and quantitation of triacylglycerols tricaprylin to tristearin was achieved by high‐performance liquid chromatography with refractive index detection and an eluent composed of propionitrile and butyronitrile (80:20, vol/vol). Peak identification was based on the logarithms of retention times, and quantitation was achieved by way of theoretical relative response factors. The validity of the response factor calculations was tested by analysis of primary standard mixtures of saturated triacylglycerols and of triacylglycerols obtained by interesterification of binary mixtures of monoacid triacylglycerols.