Abstract
The total phenols concentration in two sage macerates has been estimated by Folin-Ciocâlteau method, identified and quantified using HPLC-DAD method in order to assess the biological activity. The results for total phenols values of Folin Ciocalteau method indicate that Salvia officinalis L macerate S2, presents a higher amount of phenolic compounds than macerate S1. By HPLC-DAD method, six individual phenolic compounds were identified in sage macerates among which where cinnamic acid was found in highest concentration (652.478 mg/100g d.w. in S2 and 473.381 mg/100g d.w. in S1). The antioxidant activity of sage macerates was evaluated using DPPH Radical Scavenging test. Sage macerates exhibited high antioxidant activity, between 439.5 mg GAE /mL and 400 mg GAE /mL. Antibacterial activity of sage macerates was evaluated against 20 Gram positive and Gram negative bacterial strains isolated from clinical specimens. Both macerates showed significant but variable antibacterial activity with inhibition zones ranging from 4.97 mm (S2) to 7.28.mm (S1). The effect was stronger on Gram positive (Enterococcus, Staphyococcus) than Gram negative bacteria (Escherichia sp, Proteus sp, Klebsiella sp). Eleven metals concentrations were determined by AAS method in sage leaves; it has been found that Cd, Ni and Pb concentrations are below the detection limits.
Highlights
Quantitative Analysis of Polyphenols and Biological Activity of Sage MaceratesThe study of the antimicrobial properties and determination of the chemical constituents responsible for the biological effects of various plant components has become a priority [1316] to find new solutions in controlling pathogens and preventing infectious diseases and in exploring a natural valuable source of antioxidants
The total phenols concentration in two sage macerates has been estimated by Folin-Ciocâlteau method, identified and quantified using HPLC-DAD method in order to assess the biological activity
From all the available authentic standard used for used for determinations, only six individual phenolic compounds it were found in both sage macerates (S1, S2) (Table 5)
Summary
The study of the antimicrobial properties and determination of the chemical constituents responsible for the biological effects of various plant components has become a priority [1316] to find new solutions in controlling pathogens and preventing infectious diseases and in exploring a natural valuable source of antioxidants. In this context, the paper analyses the effect of sage macerates (S1, S2) against 20 bacterial strains isolated from clinical specimens. The content of other bioelements such as calcium, potassium, sodium, copper and zinc has been evaluated
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