Abstract
An effective ultrasonic-assisted extraction method for the separation of phenolic acids and flavonoids in Cuscuta chinensis Lam. was conducted by combining uniform design (UD) coupled with response surface methodology (RSM) and orthogonal design (OD) experiment. A sensitive and selective high-performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (HPLC-ESI-MS/MS) method was applied to quantify the sixteen active ingredients (chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, caffeic acid, hyperin, isoquercitrin, quercetin, campherol, p-coumaric acid, isorhamnetin, rutin, astragalin, and apigenin). The extraction method was optimized with respect to concentration of extraction solvent, extraction time, and ratio of liquid to solid as a consequence of getting a high sensitive and feasible method for simultaneous determination of contents of multiple components and evaluation of quality control of Cuscuta chinensis Lam. from different origins. It was also considered useful and valuable in the further study for quality control of Cuscuta chinensis Lam.
Highlights
Traditional Chinese medicines (TCMs) occupy incomparable position in the pharmaceutical industry due to their extensive activity for preventing and treating diseases
In order to obtain the optimal extraction condition, the relationship among concentration of extraction solvent (X1), extraction time (X2), and ratio of liquid to solid (X3) was researched by uniform design (U12 (12 × 6 × 6)) (Table S1 in Supplementary Materials). e quadratic polynomial stepby-step regression method and data were analyzed by Uniform Design Version 3.00 software
E data of quantitative analysis illustrated that the total content of phenolic acids and flavonoids in Cuscuta chinensis Lam. from different origins varied significantly as shown in Tables 2 and 3. e total content of phenolic acids of Cuscuta chinensis Lam. from Chongqing province was relatively higher than others, which was the lowest from Yunnan. e highest total content of flavonoids of Cuscuta chinensis Lam. of these samples was determined from Henan province, and the lowest was assessed from Zhangshu
Summary
Traditional Chinese medicines (TCMs) occupy incomparable position in the pharmaceutical industry due to their extensive activity for preventing and treating diseases. Because of their clinic application and the contribution for drug discovery, TCMs have been drawn widespread attention in the world [1]. One or very limited kind of constituent was stipulated as a marker for the quality control of herb according to the authoritative Chinese Pharmacopoeia 2015. Hundreds of constituents could be extracted from a single herb, which may exert various pharmacological functions and diverse bioactivities [2]. On account of the intimate connection with pharmacological activity, the quality of TCMs is increasingly strict and more components in TCMs should be identified and quantified grimly.
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