Abstract
Faba bean (Vicia faba L) seeds are an important source of plant protein for humans and animals. A total of 15,697 Differentially Expressed Genes (DEGs) with pathway annotation were discovered in RNA-Seq of the faba bean seeds. A total of 75 significant KEGG pathways abundance were discovered and 9 pathways were conserved within all genotypes. 41 significant pathways were found to be partially conserved within comparisons of 2 to 6 pairs of genotypes and 25 significant pathways were unique to single pairs of genotypes. There were 8 specific significant pathways discovered related to the faba bean seed Hydration Capacity trait and 9 specific significant pathways discovered related to the PSbMV seeds staining trait. The DEGs demonstrated the genetic distance between these varieties was confirmed by the breeding pedigree selection information and a PCA graph clearly illustrated the genetic distance within these genotypes.
Highlights
Faba bean (Vicia faba L) is one of the most important food and feed legume crops which provide a source of high protein for humans and animals, and it contributes to increased soil fertility through biological nitrogen fixation
There were 8 specific significant pathways discovered related to the faba bean seed Hydration Capacity trait and 9 specific significant pathways discovered related to the Pea seed-borne mosaic virus (PSbMV) seeds staining trait
The Differentially Expressed Genes (DEGs) demonstrated the genetic distance between these varieties was confirmed by the breeding pedigree selection information and a Principal component analysis (PCA) graph clearly illustrated the genetic distance within these genotypes
Summary
Faba bean (Vicia faba L) is one of the most important food and feed legume crops which provide a source of high protein for humans and animals, and it contributes to increased soil fertility through biological nitrogen fixation It is an ancient crop and is cultivated by small holder Bronze age farms in the Mediterranean region [1]. RNA-Seq technology has been used to generate genome-wide transcriptome profiles across a wide range of plants including rice [4], maize [5] [6], chickpea [7] [8], field pea [9] [10], Raphanus sativus [11], faba bean [12] [13] [14] [15] [16] and pigeonpea [17] These studies have described genome-wide gene expression levels in root, leaf, stem, T-cell and seed profiles. We applied RNA-Seq (Quantification) technology to further study Differentially Expressed Genes (DEGs) to discover the KEGG pathway enrichment information within faba bean seeds, both pathways within all seeds in common and the pathways enrichment related to the typical seed traits of Hydration Capacity and Pea seed-borne mosaic virus (PSbMV)
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