Abstract
Quantitative analysis of oxyresveratrol in different parts of Morus species by HPTLC and HPLC is described. The methods were validated for selectivity, extraction efficiency, sensitivity, accuracy, and intra-day and inter-day reproducibility. Extraction efficiency was in the range 100 ± 3.2%. Limits of detection and quantification for oxyresveratrol in plant samples were 50 and 200 ng per band, respectively, by HPTLC and 0.3 and 1.0 μg mL−1, respectively, by HPLC. The amount of oxyresveratrol was higher in stems than in leaves. It was not detected in leaves of Morus rubra L, and was detected only in roots and bark of Morus alba L. These methods, which were found to be simple and sensitive with good precision and reproducibility, were also used for analysis of oxyresveratrol in other related species and genera.
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