Abstract
Super-resolution optical microscopy has extended the scope of cell biology from the cellular level to the nanoscale, giving researchers the ability to observe the interactive behavior of single mitochondria and lysosomes[1]. However, we are still without an applicable quantitative analysis system to assist us in understanding interactive behavior of mitochondria and lysosomes under super-resolution optical microscopy. Here, we introduce an M-value to quantitatively investigate mitochondria and lysosome contact (MLC) and mitophagy under structured illumination microscopy. We found that the M-value for an MLC is typically less than 0.4, whereas in mitophagy it ranges from 0.5 to 1.0. This system permits further investigation of the detailed molecular mechanism governing the interactive behavior of mitochondria and lysosomes. [1] Q X Chen, C Z Jin, X T Shao, R L Guan, Z Q Tian, C R Wang, F Liu, P X Ling, J L Guan, L N Ji, F S Wang, H Chao, and J J Diao, Small, 1802166 (2018).
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