Abstract

The quantitative analysis of individual bile acids by gas-liquid chromatography has been improved by column oven temperature programming and by a new liquid phase, SP-2401. The method is fast; bile acids are well resolved; retention times are reproducible; detector responses are linear and sensitive to 0.1 mug: and there is little adsorption onto the liquid phase. The method has been successfully used for bile, and it has the potential for use on serum.

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