Quantitative analysis of Hyoscine in different extracts obtained from the seeds of Datura innoxia by RP- HPLC

Abstract

India has a great wealth of various naturally occurring herbal drugs which have great potential pharmacological activities. Datura inoxia is one among such ornamental herb belongs to the family Solanaceae, which bears a beautiful white, purple or yellow color, single or double blossoms flower. From ancient times continuing to the present, especially considering the Datura spp., that to be seeds, it was used in shamanistic rituals as a path to enlightenment. Solanaceae family which is of great economic importance, is one of the largest flowering plant families with about 2,300 species. Besides this, the family is also extremely important as a source of drugs in medicine such as in the treatment of skin eruptions, colds, nervous disorders, narcotic for surgical procedures, anti-spasmodic, anti-asthmatic, narcotic, antimicrobial agent and neuro-sedative, but many are poisonous when used in excess. The phytochemical investigation concluded that the leaves are rich in atropane alkaloids such as scopolamine, hyoscyamine, hyoscine, norscopolamine, meteloidine, flavonoids, cardiacs glycosides, essential oils, saponins and phenols. Today, people frequently experiment with it for the hallucinogenic effect, but the results are so unpleasant (dark visions, disorientation, amnesia, blurred vision, dry mouth, and incontinence) that they seldom recommend the experience. So in this context objective of the current review was to investigate the hyoscine content in different extract prepared with chloroform, ethyl acetate and methanol. The quantitative estimation of hyoscine in different extract was measure by RP-HPLC using PDA detector. The experimental report shows documentary evidence that, the concentration of hyoscine is maximum in chloroform and lowers in methanolic extract.