Abstract
Hydrogen peroxide (H2O2) is a representative indicator of various oxidative stresses. In this paper, we report a new silica nanoparticle platform using a combination of a multi-functional particle-based hybrid scaffold and ratiometric fluorescence probes, which can be self-calibrated for quantitative analysis. The chosen ratiometric fluorescent probe, Peroxy Lucifer 1 (PL1), was immobilized onto silica particles (PL1-SiO2) for the detection of cellular hydrogen peroxide by modulation of the internal charge transfer properties of the probe. In ratiometric analysis, a linear correlation between the hydrogen peroxide concentration and carbamate-derived emission intensities (F540/F470) was observed using PL1-SiO2. These silica particles doped with ratiometric fluorescent probes are a promising new scaffold for efficient determination of cellular hydrogen peroxide, which is a diagnostic biomarker of reactive oxygen metabolites.
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