Abstract

High performance liquid chromatography (HPLC) of desulfo-glucosinolates and of intact glucosinolates are the only two methods of analysis known at present, which can fulfil the requirements for reliable determinations of individual glucosinolates in oilseed rape and other Brassica species or cultivars. Use of HPLC requires purification of the glucosinolate-containing extract to avoid serious problems from interfering compounds. Analysis of glucosinolates in double low oilseed rape or other kinds of materials with a low content of glucosinolates requires in addition a concentration of the sample. The experimental steps in performance of these two types of analysis are nearly identical.

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