Abstract
Objective To establish a method for simultaneous determination of liquiritin, liquiritigenin, and isoliquiritinin glycyrrhizin using hydrogen nuclear magnetic resonance quantitative technology (1H-qNMR). Methodology. Deuterated dimethyl sulfoxide was used as the solvent, and dichloromethane was used as the internal standard. The probe temperature was 298.0 K, the pulse sequence was Zg30, the number of scans was 16, and relaxation delay (D1) was 10 s. Quantitative characteristic signal peaks were δ 4.891∼4.878 ppm, δ 8.187∼8.172 ppm, and δ 6.790∼6.776 ppm for liquiritin, isoliquiritin, and liquiritigenin, respectively. Results The experimental result showed that the content of flavonoids in Licorice, from Chifeng, Inner Mongolia, was the highest. Conclusion In this study, a new method for determination of three flavonoids in Licorice using 1H-qNMR was established. This experimental method has the advantages of accuracy, efficiency, and economy. It lays a foundation for the study on the determination of flavonoids content in licorice by proton nuclear magnetic resonance spectroscopy.
Highlights
Glycyrrhiza uralensis Fisch. is a popular Chinese herbal medicine derived from the dried roots and rhizomes of Glycyrrhiza uralensis Fisch. species (Leguminosae family) [1]
We have developed a quantitative Nuclear magnetic resonance (NMR) method for Angelica dahurica and Angelicae Pubescentis Radix analysis [35, 36]
There have been no reports about the simultaneous determination of liquiritin, liquiritigenin, and isoliquiritin by quantitative NMR (qNMR) method, which are the active constituents of Glycyrrhiza uralensis Fisch. erefore, the purpose of this study was firstly to simultaneously quantify the three main components liquiritin, liquiritigenin, and isoliquiritin. in Glycyrrhiza uralensis Fisch. by 1H-qNMR
Summary
To establish a method for simultaneous determination of liquiritin, liquiritigenin, and isoliquiritinin glycyrrhizin using hydrogen nuclear magnetic resonance quantitative technology (1H-qNMR). Quantitative characteristic signal peaks were δ 4.891∼4.878 ppm, δ 8.187∼8.172 ppm, and δ 6.790∼6.776 ppm for liquiritin, isoliquiritin, and liquiritigenin, respectively. E experimental result showed that the content of flavonoids in Licorice, from Chifeng, Inner Mongolia, was the highest. Is experimental method has the advantages of accuracy, efficiency, and economy. A new method for determination of three flavonoids in Licorice using 1H-qNMR was established. It lays a foundation for the study on the determination of flavonoids content in licorice by proton nuclear magnetic resonance spectroscopy
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