Abstract
During recent years our group has employed the technique of electron microprobe analysis to determine the electrolyte concentrations in various epithelial tissues. The specimen preparation is characterized by shock-freezing of small tissue pieces in liquid propane/isopentane mixtures at 77 K, cryosectioning of 1 micrometer thick serial sections at 170 K and subsequent freeze-drying at 190 K and 10-4 Pa. The analysis of the frozen dried cryosections is performed in a scanning electron microscopy which is equipped with an energy dispersive X-ray detector. The measuring conditions selected are 17-20 kV acceleration voltage and 0.1-0.5 nA probe current. For quantification, the cellular X-ray spectra are compared with those of an internal albumin standard layer. The evaluation of the characteristic X-ray intensities is performed using a computer program. Some critical points of this technique will be discussed.
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