Abstract
Quantitative Analysis of Cyclodextrins by Electrochemical Detector and Column with Core-Shell Type Filler
Highlights
Cyclodextrin (CD) is a cyclic oligosaccharide, which is α (1 → 4) glycosidically linked to D-glucopyranose
Using 0.1 mol/L NaOH/0.1 mol/L CH3COONa=1/1 as the mobile phase, peaks for αCD and βCD were not observed with either S-30/70=St/DVB-5TMDAH or S-40/60=St/DVB-5TMA columns (Figure 3)
The measurement of CDs was made possible by using a sodium hydroxide solution as the mobile phase and a column containing coreshell type filler reacted with an amine
Summary
Cyclodextrin (CD) is a cyclic oligosaccharide, which is α (1 → 4) glycosidically linked to D-glucopyranose. Detectors and instruments such as evaporation light scattering detectors (ELSD), refractive index detectors (RI), and mass spectrometers (MS) have been used for CD quantification [2,3,4,5]. These methods are not suitable for low concentration measurements due to their large quantitative limit (about 50 mg/mL) [6]. When a basic solvent is used for the mobile phase, the alcoholic hydroxyl groups of saccharides are dissociated, and the ECD detects saccharides present as ionic molecules in anion exchange chromatography. The advantages of the ECD are: 1) it can be used for substances having alcoholic hydroxyl groups, 2) it has high sensitivity, and 3) it does not require additional sample preparation such as derivatization
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