Abstract
A method for the quantitative analysis of the molecular species of glycerolipids present In biological samples has been described. 1,2-Diacyl- sn-glycerol, either isolated from biological samples or enzymatically generated from phosphoglycerides, is benzoylated at the sn-3 position and then subjected to reverse-phase (C 18-silica) HPLC to separate the molecular species of different hydrophobicitles. An internal standard (1,2-distearoyl- sn-glycerol) is used to identify and quantify the various species eluted from the reverse-phase column. Examples are given for the quantitative analysis of molecular species and precursor-product relationships of glycerolipids generated In SK-N-SH neuroblastoma cells after stimulation of the cell-surface muscarinic acetylcholine receptors.
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