Abstract
Objective -To determine the content of caffeine in the beverages like green tea, black tea, and soft drink which are commercially available in the local market of Hapur with the help of a UV Visible spectrophotometer. Method- To find out the content of caffeine, six different brands of green tea, black tea, and soft drink were purchased from the local market in Hapur. The caffeine was extracted from them using chloroform as an extractant and assessed qualitatively and quantitatively with the help of a UV Visible spectrophotometer. The maximum absorbance of caffeine in chloroform occurs at 273 nm. The standard solutions of caffeine from the range 2-30 ppm were prepared in the chloroform, show the linearity with the correlation coefficient of 0.99. From the calibration curve, the concentration of caffeine was determined in various brands. It is observed that black tea contains the maximum caffeine content followed by green tea and soft drinks. Findings- In this study the maximum caffeine content find out was (45.6 mg/g) in the Nice black tea sample and the minimum (0.161 mg/ml) in Pepsi soft drink sample. Novelty-The caffeine content reported here is higher than that of previous studies may be due to the modified approach [treatment temperature (90-1000C) and longer brewing time (05 minutes) in this study. The use of the UV-Visible spectrophotometer is also an alternative to the HPLC technique, thereby the working cost could be brought down. Keywords: Caffeine; brewing time; beverages; UV visible spectrophotometer; extraction
Highlights
Caffeine (1,3,7-trimethylxanthine) is a purine alkaloid and is largely present in several beverages
The amount of caffeine in soft drinks varies among brands which are regulated by the US Food and Drugs Administration (FDA) to no more than 6 mg/oz fluid or 200 mg/L
The experimental caffeine level in the various samples is given in the Table 2 and compared with value of caffeine reported in earlier studies
Summary
Caffeine (1,3,7-trimethylxanthine) is a purine alkaloid and is largely present in several beverages. It becomes essential to quantify the caffeine in various food items. The beverages such as tea, coffee and soft drinks are the most widely consumed in the world have caffeine. The amount of caffeine in soft drinks varies among brands which are regulated by the US Food and Drugs Administration (FDA) to no more than 6 mg/oz fluid or 200 mg/L. Analysis of caffeine is required to ensure proper caffeine levels in beverages and to meet regulatory standards [1]. It is important to monitor caffeine in beverages and food by establishing a more precise, simple, fast and low-cost analytical method in order to study its physiological
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