Quantitative Analysis of Abamectin, Albendazole, Levamisole HCl and Closantel in Q-DRENCH Oral Suspension Using a Stability-Indicating HPLC-DAD Method.

Hazim M Ali,Lobna Mohammed Abd Elhalim,Mohammed M Ghoneim,Mohammed Gamal

doi:10.3390/molecules27030764

Hazim M Ali, Lobna Mohammed Abd Elhalim + Show 2 more

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https://doi.org/10.3390/molecules27030764

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Abstract

Combination therapy of many anthelmintic drugs has been used to achieve fast animal curing. Q-DRENCH is an oral suspension, containing four different active drugs against GIT worms in sheep, commonly used in Australia and New Zeeland. The anti-parasitic drugs are Albendazole (ALB), Levamisole HCl (LEV), Abamectin (ABA), and Closantel (CLO). The main purpose of this study is to present a new simultaneous stability-indicting HPLC-DAD method for the analysis of the four drugs. The recommended liquid system was 1 mL of Triethylamine/L water, adjusting the pH to 3.5 by glacial acetic acid: acetonitrile solvent (20:80, v/v). Isocratic elusion achieved the desired results of separation at a 2 mL/min flow rate using Zorbax C-18 as a stationary phase. Detection was performed at 210 nm. The linearity ranges were 15.15 to 93.75 μg/mL for ALB, 25 to 150 μg/mL for LEV, 30 to 150 μg/mL for ABA, and 11.7 to 140.63 μg/mL for CLO. Moreover, the final greenness score was 0.62 using the AGREE tool, which reflects the eco-friendly nature. Moreover, the four drugs were determined successfully in the presence of their stressful degradation products. This work presents the first chromatographic method for simultaneous analysis for Q-DRENCH oral suspension drugs in the presence of their stressful degradation products.

Highlights

Co-administration of ALB and Levamisole HCl (LEV) causes the fast killing of the parasites if their concentrations exceed the minimum therapeutic effect in animal plasma, it cannot protect the animals from reinfection
For LEV, the highest degradation was detected in the photodegradation conditions (56.35%), while the lowest degradation was identified in the H2 O2 medium (12.96%)
Because the first goal of this work was to develop a simple RP-HPLC method in pharmaceuticals for regular QC use, the proposed RP-HPLC method was not tested with biological fluids from animals

Summary

Introduction

Gastrointestinal infection with nematodes is one of the main causes of financial losses in sheep breeding [1,2]. Due to the increased rate of parasite resistance, a combination therapy of many anthelmintic drugs has been used to achieve fast animal curing [2]. QDRENCH [3] is an oral suspension, containing four different active drugs with different pharmacological activities against GIT worms in sheep, commonly used in Australia and. The anti-parasitic drugs are Albendazole (ALB), Levamisole HCl (LEV), Abamectin (ABA), and Closantel (CLO); see Figure 1. Co-administration of ALB and LEV causes the fast killing of the parasites if their concentrations exceed the minimum therapeutic effect in animal plasma, it cannot protect the animals from reinfection

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