Abstract
Cheongsangbangpung-tang (CSBPT) is a traditional herbal medicine that has been used in many regions of Asia, including Korea, China, and Japan, for the treatment of purulent inflammation and eczema on the face. In this study, a method for the simultaneous analysis of 18 marker components, geniposide (1), coptisine chloride (2), prim-O-glucosylcimifugin (3), berberine chloride (4), liquiritin apioside (5), liquiritin (6), ferulic acid (7), narirutin (8), 5-O-methylvisammisoide (9), hesperidin (10), arctigenin (11), baicalin (12), oxypeucedanin hydrate (13), wogonoside (14), baicalein (15), arctiin (16), glycyrrhizin (17), and pulegone (18), was developed for quality control of CSBPT. The novel approach, which is based on high-performance liquid chromatography (HPLC) separation coupled with photodiode array detection, was verified by the assessment of linearity, limit of detection, limit of quantification, accuracy, recovery, and precision. Analysis of CSBPT by using the established assay revealed that compounds 1–18 were present in concentrations of 0.27–18.31 mg/g.
Highlights
Oriental medical prescriptions consisting of a combination of two or more medicinal herbs and containing many components have been widely used in Asian countries, such asKorea, China, and Japan, to enhance human health and prevent various diseases
There have been many simultaneous analysis methods reported for the quality assessment of each constituent herbal medicine of CSBPT based on high-performance liquid chromatography (HPLC), gas chromatography, and liquid chromatography–mass spectrometry [9,10,11,12,13,14,15,16,17,18,19,20]
For the selection of marker components for the quality evaluation of CSBPT, we analyzed and confirmed the main components of 12 raw herbs as follows: prim-Oglucosylcimifugin and 5-O-methylvisammioside from Saposhnikoviae Radix; imperatorin, isoimperatorin, oxypeucedanin, and oxypeucedanin hydrate from Angelicae Dahuricae; arctigenin and arctiin from Forsythiae Fructus; platycodin D and platycodin D2 from
Summary
Oriental medical prescriptions consisting of a combination of two or more medicinal herbs and containing many components have been widely used in Asian countries, such as. CSBPT was first recorded in Gogeumuigam of Gong Shin in the Ming Dynasty of China, and it has been included in Dongeuibogam of Heo Jun in the Joseon Dynasty of Korea [1,2] This prescription consists of a combination of 12 medicinal herbs: Saposhnikoviae Radix, Angelicae Dahuricae Radix, Forsythiae Fructus, Platycodonis Radix, Scutellariae Radix, Cnidii Rhizoma, Schizonepetae Spica, Gardeniae Fructus, Coptidis Rhizoma, Aurantii Fructus Immaturus, Menthae Herba, and Glycyrrhizae Radix et Rhizoma, in 2.5:2.7:2.7:2.7:2.3:2.3:1.7:1.7:1.7:1.7:1.7:1.0 ratio [2]. There have been many simultaneous analysis methods reported for the quality assessment of each constituent herbal medicine of CSBPT based on high-performance liquid chromatography (HPLC), gas chromatography, and liquid chromatography–mass spectrometry [9,10,11,12,13,14,15,16,17,18,19,20]. From Coptidis Rhizoma, narirutin (8) and hesperidin (10) from Aurantii Fructus Immaturus, and liquiritin apioside (5), liquiritin (6), and glycyrrhizin (17) from Glycyrrhizae Radix et Rhizoma
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