Quantitative analysis for phenolic acids by thin-layer chromatography

Abstract

A method is described by which phenolic acid compounds occurring in plant material can be separated on a polyamide column after suitable hydrolysis. The mixture of phenolic acids was also separated on silica gel G thin-layer plates, and the individual phenolic acids were determined spectrophotometrically. Phenolic acid compounds were hydrolysed by a technical enzyme or by treatment with 1% barium hydroxide solution, followed by 1% sulphuric acid. The method was applied successfully to fruit and vegetables.