Abstract
A method for quantification of unlabeled α-tocopherol and the deuterated tocopherols, RRR-α-5-(CD3)-tocopherol (d3RRR) and all rac-α-5,7-(CD3)2 tocopherol (d6all-rac) in plasma by HPLC–tandem mass spectrometry (LC-MS/MS) has been developed. Deuterated and unlabeled α-tocopherols were separated by HPLC and were detected by positive ion multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with a heated nebulizer–atmospheric pressure chemical ionization interface, following routine extraction of vitamin E from plasma. The accuracy and precision were evaluated by replicate analysis of standards and samples. Human plasma samples, which were obtained at different times after the subject had consumed a capsule containing 1:1 ratio of d3RRR and d6all rac-α-tocopheryl acetates, were analyzed with this method. Plasma deuterated α-tocopherols measured by LC-MS/MS followed the same pattern as previously demonstrated by GC-MS measurement, without requiring an extra derivitization step. The detection limit was 10 pmol for each form of α-tocopherol injected.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
