Abstract
A chromatographic procedure is used to obtain data on amounts of the oxytocin-neurophysin (OT-NP, or “A”) and its more completely processed form (OT-NP′, or “B”), which lacks the carboxyl-terminal glutamic acid residue of the A form. Measurements of B + A and B/(B + A) are made for single neurohypophyses under varying physiological conditions, in release obtained by perfusion of isolated neurohypophysis with saline containing increased external potassium concentrations, and in isolated neural lobe nerve endings and nerve swellings. The chromatographic procedure is also combined with injection of [ 35S]cysteine over the supraoptic nucleus. This approach is used to obtain the in vivo A to B conversion rate, and to investigate the trafficking of newly synthesized secretory granules in different axonal compartments. The manner with which the conversion rate changes with secretory activity is investigated by comparing the conversion rate in control animals to that in animals subjected to osmotic stress. Generalized computer models of peptide processing, storage, and release in neurons are used to test various hypotheses of the control of secretory granule movements in the neurohypophysis for sufficiency in explaining the changes in B + A and B/(B + A) with changing physiological state.
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