Abstract
Frying oils collected in restaurants were fractionated into a polar and a non-polar fraction by the Iatroscan thin-layer chromatography-flame ionization detection (TLC-FID) system on Chromarod S II using hexane diethyl ether-acetic acid (97:3:1) as the solvent system. The FID responses for Iatroscan analyses of the polar and the non-polar fraction isolated from a frying oil by column chromatography on a 5% hydrated silicic acid were studied at Chromarod load levels ranging from 1 to 16 μg, relative to methyl heptadecanoate as the internal standard. The correction factors were relatively constant in the range 10–16 μg, but increased in the range 1–5 μg. The amount of polar material in ten commercial frying oil samples was quantitated by the Iatroscan TLC FID technique. Good correlations were found between the results and data obtained by column chromatography and silica gel Sep-Pak cartridges.
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