Abstract
Publisher Summary Recent developments in molecular biology and the isolation of the two different but closely related genes for vasopressin (VP) and oxytocin (OT) made it possible to develop methods for the quantitation of the messenger RNA (mRNA) levels of these two neuropeptides. These mRNA levels are a reflection of the gene transcription rates, and they also provide a useful index for the biosynthetic capacity of neurons to synthesize a given peptide. The use of the vasopressin and oxytocin systems for studies on neuropeptide gene regulation is very advantageous, because the vasopressinergic and oxytocinergic neurons are clearly grouped into distinct nuclei of the brain that are linked to peripheral or central functions of these neuropeptides. Just as the solution hybridization assay, dot-blot analysis can provide accurate levels of VP mRNA and OT mRNA but not information on the nature and integrity of the RNA. Although the dot-blot assay requires a more extensive deproteination of RNA samples and takes more time to obtain results, it has the advantage that it has a higher sensitivity due to the steep standard curve.
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