Abstract
Mass fragmentographic studies of estrogens in urine samples in pregnancy revealed that a sensitivity from at least 100 up to 10,000 times as compared to conventional gas chromatography with hydrogen flame ionisation detector can be achieved. The specificity of the determination is also considerably increased. Even amounts less than 10 pg can sometimes be detected and analysed if optimal conditions are employed. This means that 9 estrogens may be analysed in a 1–5 μ1 sample of late pregnancy urine and that only about 50 μl is needed to estimate all 12 estrogens included in the method. Mass fragmentograms of 12 naturally occuring estrogens are presented.
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