Abstract
Medical treatment utilizing non-thermal plasma is based on the production of reactive oxygen species (ROS) and their interactions with biomatters. On the basis of empirical data from practices, plasma treatment has been planned with regard to the setup of a plasma generator’s parameters, including gas combination, gas-flow rate, and applied voltage. In this study, we quantitated plasma treatment in terms of the plasma dose on the target matter, which can be contrasted with the radiation dose to targets under radiation exposure. We measured the OH radical production in cell culture medium and intracellular ROS production from plasma treatment in comparison with those from X-ray exposure. The clonogenic cell deaths from plasma and X-ray exposures were also compared. In plasma treatment, the clonogenic cell death was better predicted by intracellular ROS production rather than by medium OH production.
Highlights
Medical treatment utilizing non-thermal plasma is based on the production of reactive oxygen species (ROS) and their interactions with biomatters
Medium OH and intracellular ROS productions were considered as common indices for the bioeffects of radiation and plasma
The equivalent X-ray dose to each operational setup of atmospheric pressure plasma jet (APPJ) device was defined as the X-ray dose that would induce comparable radical production or the comparable fraction of clonogenic cell death to plasma treatment
Summary
Medical treatment utilizing non-thermal plasma is based on the production of reactive oxygen species (ROS) and their interactions with biomatters. Non-thermal plasma and ionizing radiation operate a common pathway to cell-killing effect, that is, ROS production. Plasma treatment is prescribed in terms of the operational setup of the source device with regard to gas combination, gas-flow rate, applied voltage, and treatment duration, which contrasts with the radiation treatment prescribed in terms of radiation dose. We evaluated the effects of individual parameters, such as gas-flow rate, applied voltage, and treatment duration, of a plasma production device on the radical production and clonogenic death of in vitro cells. We sought the radiation dose levels that cause the comparable radical productions and clonogenic cell deaths with the plasma exposures under different parametric combinations in operation of a plasma source device
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