Abstract
Oudin's single diffusion method in agar gel has been applied to quantitate the group specific component in human sera. The method is highly reproducible in double tests of the same serum specimens, and its sensitivity measured in series of serum protein dilutions has been found to be satisfactory.By the method presented, the Gc concentrations have only been determined in relative values.The concentration of the group specific component has been measured in the serum from each of 50 healthy adults. The values varied between 80 and 140 per cent of the chosen standard.The present method may be of help in attempts to solve the question of the biological function of the group specific component.
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