Abstract
Summary An enzyme-linked immunosorbent assay (ELISA) was calibrated and used for quantitation of the folate binding protein in human cerebrospinal fluid. The precision of the assay in terms of the coefficient of variation was 6.3% intra-assay (10 replicate single determinations) and 4.8% inter-assay (8 duplicate determinations). The concentration of folate binding protein in cerebrospinal fluid specimens from 20 patients with no complaints or diseases of neurological type was 0.14-0.38, 0.24 nmol FBP/I (range and median value). The gelfiltration profiles of radioligand bound and immunoreactive folate binding protein were identical, both consisting of one major peak (M, '" 25000) and one minor peak (M, '" 1 00000).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
