Quantitation of human chorionic gonadotropin in urine using the slide immunologic test for pregnancy

Abstract

1.1. The use of the slide immunologic test in a semiquantitative procedure for determining HCG titers in urine has been described. This method is simple, reliable, and rapid. The test can be performed in one hour.2.2. The sensitivity and accuracy of this method have been compared with those of the Friedman test and the results found have been good.3.3. The sensitivity of the immunologic test described is approximately 5 I.U. per milliliter, as determined by the use of the Second International Standard for human chorionic gonadotrophin.4.4. The geometric mean of titers observed in 295 urine samples obtained at varying times throughout pregnancy is presented. In the practical use of this test, a titer of 1:64 (320 I.U. per milliliter) is considered the high limit of normal during the first trimester of pregnancy.5.5. The potential use of this serologic test in the diagnosis of abnormal conditions like hydatidiform moles and chorionepitheliomas is discussed.