Quantitation of Hepatitis B Viral Markers in a Dental School Population

Abstract

Ample evidence exists that dentists and dental personnel are vulnerable to hepatitis B disease. Epidemiologic studies, which provide a further understanding of the prevalence, infectious nature, and risks of transmission of the hepatitis B virus, are particularly significant in a dental school environment because of the insidious nature of the virus. Screening should be done for anti-HBs because it is the most reliable marker for hepatitis B infection and is generally believed to confer immunity. However, because no experimental data are available concerning the amount of antibody needed for protection, quantitation of anti-HBs levels in human serum or gingival crevicular fluid would be the first step in ultimately correlating antibody titers to the immune process. In this study, we present quantitative data on the relative antibody titers in a dental school population. Our findings may be of importance for monitoring the effectiveness of vaccination, for improving safety guidelines in dental clinics, and for developing new and rapid oral diagnostic screening methods. Recently, a new method using gingival crevicular fluid has been developed; it permits identification of not only antibody, but also of hepatitis B surface and e antigens (L. Andors, and associates, unpublished data).