Abstract
Following the application of 10-4 molar tritium labeled diisopropyl fluorophosphate (3H-DFP) to fresh or glutaraldehyde fixed rat liver, fluorophosphate-reactive (FPR) sites within the hepatocytes were measured using quantitative electron microscope autoradiography (EMARG). Based on the distribution of autoradiographic silver grains, most of the FPR sites were concentrated in the rough and smooth endoplasmic reticulum and associated ground cytoplasm. Cytoplasmic granules, comprising autophagic and residual granules also contained FPR sites. The average concentration of sites within each of these cytoplasmic structures was obtained from combined morphometric and grain density analyses of the EMARGs and light microscope sections of the same epoxy embedded liver specimens.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
