Abstract
Factor VIII related antigen (FVIIIRAG) was studied by kinetic analysis of light scattering in a laser nephelometer. Plasma samples were obtained from normal donors, from patients with haemophilia A and from patients with von Willebrand's disease. Samples were reacted in aqueous buffer solutions with antisera to human factor VIII related antigen and the characteristics of the antigen-antibody reactions were evaluated in a helium-neon laser nephelometer. The formation of immunocomplexes in mixtures of antigen and antibody were followed in the laser nephelometer by progressive measurement of light scattered at different times. An assay method was developed which facilitated the measurement of factor VIII related antigen at a minimum level of 0.03 units per ml with an incubation period of 300 minutes. Quantitative values of factor VIII related antigen measured by this method were similar to the assay results obtained by the Laurell rocket method but differences in the kinetics of immunoprecipitate formation were observed with different plasma samples. It is suggested that the differences in immunoreaction detected by the laser nephelometer may be due to different polymeric forms of factor VIII related antigen in plasma.
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