Abstract
A sensitive and specific double antibody radioimmunoassay has been developed capable of measuring gonadotropin releasing hormone (GnRH) in unextracted human plasma. Parallelism between synthetic GnRH and unextracted plasma was demonstrated. Synthetic GnRH could be quantitatively recovered when added to plasma. Maximal plasma concentrations (ng/ml) occured within 2m–4 min. of intravenous injection of 50 or 100μg GnRH to 2 adult males and 6 adult females: 50μg, 7.2 ± 1.7 (mean ± S.E.); 100μg dose, 13.4 ± 3.1. The resulting composite disappearance curve appeared to be the result of 2 linear components, the first corresponding to a tjg of 3.6 min., and the second to a t½ of 19.3 min. Preliminary measurements of endogenous concentrations (pg/ml) of immunoreactive GnRH were: children, 30.7 ± 7.7, adult females during the follicular phase of the menstrual cycle, 69.6 ± 12.3, and adult males, 67.9 ± 17.3 (mean ± S.E.).
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