Abstract
A simple solid phase extraction (SPE) technique combined with gas chromatography-mass spectrometry (GC/MS) operated in selected ion monitoring (SIM) mode is described for quantitation of cocaine and cocaethylene in small samples (250 μl) of rat whole blood. Use of ( N-[ 2H 3C])-cocaine and (N- [ 2H 3C])-cocaethylene internal standards resulted in high sensitivity and selectivity for this analytical method. Analysis was performed using a Hewlett-Packard 5890 GC equipped with a 7673A Automatic Liquid Sampler linked to a Hewlett-Packard 5972 Mass Selective Detector. Separation of analytes was accomplished on a cross-linked methyl silicone gum capillary column (Ultra 1: 12m × 0.2mm (i.d.) × 0.33 μm). Linearity was established over a wide range of concentrations (5.0–2000.0 ng ml −1) with good precision. Limits of detection (LOD) were 1.0 and 2.0 ng ml −1 for cocaine and cocaethylene, respectively. This analytical method was designed for use in pharmacokinetic experiments studying the formation of cocaethylene following ethanol pretreatment in rats administered cocaine.
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