Quantifying Trophoblast Function Using the xCELLigence System.

Abstract

Analyses of trophoblast behavior in vitro are widely used to investigate and understand the trophoblast dysfunction associated with pregnancy pathologies. Key behaviors critical for trophoblast function include adhesion, proliferation, migration, and invasion. Traditionally, these behaviors have been measured using time-consuming single timepoint or endpoint assays. xCELLigence is a breakthrough technology that utilizes gold electrodes embedded in cell culture plates to collect electrical impedance measurements and monitor cell attachment, spreading, movement, and growth. It enables trophoblast functions to be quantitatively monitored in real-time, providing new insights into trophoblast behavior.