Quantifying the effectiveness of ultraviolet-C light at inactivating airborne Mycobacterium abscessus

Abstract

Mycobacterium abscessus (MABS) group are environmental organisms that can cause infection in people with cystic fibrosis (CF) and other suppurative lung diseases. There is potential for person-to-person airborne transmission of MABS among people with CF attending the same care centre. Ultraviolet light (band C, UV-C) is used for Mycobacterium tuberculosis control indoors; however, no studies have assessed UV-C for airborne MABS. To determine whether a range of UV-C doses increased the inactivation of airborne MABS, compared with no-UVC conditions. MABS was generated by a vibrating mesh nebulizer located within a 400L rotating drum sampler, and then exposed to an array of 265nm UV-C light-emitting diodes (LED). A six-stage Andersen Cascade Impactor was used to collect aerosols. Standard microbiological protocols were used for enumerating MABS, and these quantified the effectiveness of UV-C doses (in triplicate). UV-C effectiveness was estimated using the difference between inactivation with and without UV-C. Sixteen tests were performed, with UV-C doses ranging from 276 to 1104μWs/cm2. Mean (±SD) UV-C effectiveness ranged from 47.1% (±13.4) to 83.6% (±3.3). UV-C led to significantly greater inactivation of MABS (all P-values ≤0.045) than natural decay at all doses assessed. Using an indoor model of the hospital environment, it was estimated that UV-C doses in the range studied here could be safely delivered in clinical settings where patients and staff are present. This study provides empirical in-vitro evidence that nebulized MABS are susceptible to UV-C inactivation.